尿路病原性大腸菌の接触依存性増殖阻害蛋白質（CdiA-CT）は隣接細菌内でシステイン生合成因子CysKによって活性化されます。その結果、隣接細菌内のtRNAを切断し、細菌の増殖を阻害します。今回、CysK:CdiA-CT:tRNAの三者複合体のCryo-EM単粒子構造解析、機能解析から,CysK:CdiA-CT複合体によるtRNA分子認識、切断の分子機構を明らかにしました。”Mechanism of activation of contact-dependent growth inhibition tRNase toxin by the amino acid biogenesis factor CysK in the bacterial competition system.” Nucleic Acids Res. (2024) doi.org/10.1093/nar/gkae735

The contact-dependent growth inhibition protein (CdiA-CT) of uropathogenic Escherichia coli is activated by the cysteine biosynthesis factor CysK within neighboring bacteria. As a result, it cleaves tRNA in the neighboring bacteria, thereby inhibiting their growth. In this study, we elucidated the molecular mechanism of tRNA recognition and cleavage by the CysK:CdiA-CTcomplex through Cryo-EM single-particle structural analysis and functional analysis of the CysK:CdiA-CTternary complex. “Mechanism of activation of contact-dependent growth inhibition tRNase toxin by the amino acid biogenesis factor CysK in the bacterial competition system.” Nucleic Acids Res. (2024) doi.org/10.1093/nar/gkae735